Fig. 4: Control of reaction time and cross reactivity tests. | Nature Communications

Fig. 4: Control of reaction time and cross reactivity tests.

From: Diagnosis and prognosis of myocardial infarction on a plasmonic chip

Fig. 4

a Fluorescence mapping results and b corresponding calibration curves with the reaction time of 150, 60, and 30 min for cTnI (0.01–0.08 ng mL−1). All experiments were conducted with n = 3; mean ± s.d. c Fluorescence images showing only one row of spots (cTnI) emitting bright fluorescence signals when a multiplexed antibody chip (two rows, two different antibodies against biomarkers labeled at the left of the image with different concentrations) were incubated in a solution containing a mixture of the two antigens, followed by incubation in a solution containing only one of the corresponding detection antibodies without the other one. d Averaged fluorescence intensity of each row in (c). All experiments were conducted with n = 3; mean ± s.d. Source data are provided as a Source Data file.

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