Fig. 2: Dynamics of CRISPRi library during photoautotrophic growth. | Nature Communications

Fig. 2: Dynamics of CRISPRi library during photoautotrophic growth.

From: Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Fig. 2

a Clustered sgRNAs by similarity of log2 fold-change over time. Rows represent time-points sampled after induction. (L100—light with 100 µmol m−2 s−1, L300—light with 300 µmol m−2 s−1, LD—light-dark cycle). All log2 fold-change values were calculated from averages of four replicate cultivations. Symbols: plus, induced, minus, non-induced, star, cluster 5 is a combination of two unchanged clusters. b Log2 fold-change of individual sgRNAs over the course of each cultivation. Experiment run time was normalized to number of cell generations estimated from population growth rate. c Enriched gene-ontology (GO) terms for the four clusters (1–4) showing sgRNA depletion; p-value—Fisher’s exact test with elimination (see Methods). Source data are provided as a Source Data file.

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