Fig. 4: Transcriptomics of faster-growing mutants.

a Enrichment of faster-growing sgRNA mutants in library competition (turbidostat) experiment at L300 (300 µmol photons m⁻² s⁻¹), log₂ fold-change of read count over time (n = 2 sgRNAs). b Volcano plot representation of transcriptomics data from four reconstructed sgRNA mutants. Shows log₂ fold-change for gene expression compared to the control strain (sgRNA-NT0), against adjusted p-value for each gene. Gray—non-significantly different genes, pink—significantly different genes (threshold: negative log₁₀ p-value ≥ 2; absolute log₂ fold-change ≥ 1). The p-value for three sgRNA mutants was outside the plotting region and was restricted to −log₁₀ of 50 for visibility. c Heat map representation of transcriptomics data from four reconstructed sgRNA mutants. Shows all 305 genes that were significantly different in at least one of the four mutants, clustered into two different groups based on similarity of gene expression. d Significantly different genes are sorted by Cyanobase pathways. Genes of particular interest were highlighted (see text for details). Blue and orange indicates negative and positive log₂ fold-change, respectively. Size of symbols increases with increasing absolute change. Source data underlying Fig. 4b, d are provided as a Source Data file.