Fig. 6: SgRNA clones with increased L-lactate production.
Mutants with potentially increased L-lactate productivity were discovered using a fluorescence-activated droplet sorting assay. Seven selected mutants were reconstructed, cultivated, and product titer determined in µmol per liter, and µmol per liter per biomass (OD720 nm). a Productivity of selected mutants cultivated in axenic shake-flask cultures (n = 2, each strain assayed in two independent replicates). L-lactate concentration was measured after 24 and 48 h. NT0 - control sgRNA with no target site in Synechocystis genome. *—significant with p ≤ 0.05, two-sided student’s t-test, pcnB, p = 0.016, gltA, p = 0.021. Bars are average values and individual values are shown as points. b A subset of two mutants was cultivated in axenic ‘photonfluxostat’ cultures (light intensity proportional to cell density, 1000 µmol photons m−2 s−1 OD720 nm−1, n = 2, each strain assayed in 2 separate experiments). L-lactate concentration was measured after 18 and 30 h. Bars are average values and individual values are shown as points. Source data are provided as a Source Data file.
