Table 1 Selection of sgRNA clones enriched by droplet sorting.

From: Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

sgRNA

Appearances

Effect on growth

Gene function

gdhA_25*

4

None

Glutamate dehydrogenase

ssr2016_62*

3

Impaired

Ferredoxin:plastoquinone reductase, cyclic electron flow

gltA_41*

3

Impaired

Citrate synthase

pntB_19

3

None

Transhydrogenase subunit

pcnB_64*

3

Impaired

Nucleotidyltransferase

serA_10*

2

Impaired

Phosphoglycerate dehydrogenase

gltB_3*

2

Impaired

Glutamate synthase

ndhD2_15

2

None

NDH-1 complex subunit, cyclic electron flow

sdhB_26*

2

None

Succinate dehydrogenase

Entry85_13

2

None

atpA (ATP synthase) asRNA

pstC_41

2

None

Phosphate transporter

slr0453_10

2

None

Phosphoketolase

  1. Note: Mutants were identified from a CRISPRi library based on L-lactate productivity. Appearance is the number of sorting runs (out of four) where that clone had an enrichment factor of at least 2 (normalized abundance in sorted population/normalized abundance in unsorted population). Clones where growth was impaired are denoted. These clones were significantly depleted in the L300 dataset after four generations (ssr2016_62, padj = 1.6e−94; gltA_41, padj = 3.1e−53; pcnB_64, padj = 1.5e−58; serA_10, padj = 3.6e−21; gltB_3, padj = 0.015; multiple-hypothesis adjusted p-values calculated by DESeq2). An asterisk denotes sgRNA mutants selected for reconstruction and validation. Source data are provided as a Source Data file.
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