Fig. 2: Cfz induces phosphorylation of splicing factors in a dose-responsive manner. | Nature Communications

Fig. 2: Cfz induces phosphorylation of splicing factors in a dose-responsive manner.

From: Proteasome inhibitor-induced modulation reveals the spliceosome as a specific therapeutic vulnerability in multiple myeloma

Fig. 2

a–d Volcano plots of log2-transformed ratios of phosphosite abundances between a MM.1S treated with 18 nM Cfz, b 10 nM Cfz, or d 10 μM melphalan compared to DMSO and c AMO-1 with 15 nM Cfz compared to DMSO. Significant upregulated sites are in red, while downregulated are in blue (>2-fold change, p < 0.05). SRSF-related sites are in orange. Circle size corresponds to summed SILAC intensities. e Top-ranked GO terms for genes with significantly upregulated phosphosites in MM.1S cells treated with 18 nM Cfz. f Top ranked KSEA-activated kinases (with at least 5 substrates) for MM.1S treated with 18 nM Cfz (top panel) and 10 μM melphalan (bottom panel).

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