Fig. 5: Depletion of Treg cells increases localised proinflammatory cytokine expression.
From: Regulatory T cells confer a circadian signature on inflammatory arthritis
a Two daily injections of DTX at ZT6 led to significant depletion of Tregs 24–36 h after the last injection. b Progression of arthritis (as assessed by hind paw swelling) in control mice (n = 13) and in mice with depleted Tregs (n = 8), two-sided paired T tests. c Quantification of Tregs in arthritic control (n = 6) and Treg depleted (n = 6) animals, two-sided unpaired T-tests. Data are presented as mean values ± SEM. d Quantification of inflammatory cell populations within the inflamed joints in arthritic control (n = 6) and Treg-depleted mice (n = 6), one-way ANOVA. Data are presented as mean values ± SEM. e QPCR analysis of inflammatory genes (normalised to gapdh) within the joints of arthritic control (n = 11) and Treg-depleted (n = 9) mice culled at ZT18. Values were normalised to expression in control animals without arthritis, two-sided unpaired t tests. Data are presented as mean values ± SEM. f QPCR analysis of inflammatory genes (normalised to gapdh) within the joints of arthritic control (n = 7) and Treg-depleted (n = 11) mice culled at ZT6. Values were normalised to expression in control animals without arthritis. Data are presented as mean values ± SEM. g Effect of depletion of Tregs on IL-1β expression by joint monocytes (control n = 6; Treg depleted n = 5), two-way ANOVA and post hoc Bonferonni. Graph shows individual data points and mean values. In all panels statistical significance is shown as *p < 0.05, **p < 0.01 and ***p < 0.005. Source data are provided as a Source Data file.
