Fig. 6: Warm temperature and shade elicit distinct photobody dynamics.

a Maximum-projection, deconvolved fluorescence microscopy images showing representative steady-state patterns of phyB-GFP in cotyledon epidermal-cell nuclei of 4-day-old PBG seedlings grown at 21 °C in 10 µmol m⁻² s⁻¹ continuous R light (21 °C, Rc), at 21 °C in 10 µmol m⁻² s⁻¹ R light supplemented with 10 µmol m⁻² s⁻¹ FR light (21 °C, shade), and at 27 °C in 10 µmol m⁻² s⁻¹ R light. b Quantification of the numbers of total, Nuo, and nonNuo PBs or foci per nucleus in cotyledon epidermal-cell nuclei of the PBG seedlings described in a. The boxes represent from the 25th to the 75th percentile and the bars equal the median values. Asterisks represent statistically significant differences in PB number (unequal variances t test, P ≤ 0.0001, n ≥ 6). c Distribution of the volume of PBs or foci in cotyledon epidermal-cell nuclei of the PBG seedlings grown in the indicated shade, 21 °C, and 27 °C conditions described in a. Error bars represent s.d. d Schematic illustration of the model of temperature-dependent PB dynamics. The circles filled with different colors represent PBs with a gradient of distinct thermostabilities. The circle with a dotted line represents the nucleolus. The most thermostable PB persists in all temperatures, whereas the less thermostable ones form only in lower temperatures. As a result, temperature increases progressively trigger the disassembly of individual PBs. The source data underlying the PB measurements in b, c are provided in the Source Data file.