Fig. 7: Deletion of the Ntrk2 gene in PVH neurons projecting to the LPBN leads to hyperphagia and obesity. | Nature Communications

Fig. 7: Deletion of the Ntrk2 gene in PVH neurons projecting to the LPBN leads to hyperphagia and obesity.

From: TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits

Fig. 7

a Deletion of the Ntrk2 gene in PVH neurons projecting to the LPBN. AAV2retro-hSyn-FLPo-T2A-GFP (200 nl) and either AAV2-Ef1a-fDIO-mCherry (control) or AAV2-Ef1a-fDIO-mCherry-P2A-Cre (150 nl) were bilaterally injected into the LPBN and PVH of Ntrk2lox/lox mice, respectively (a1). GFP marks injection sites of AAV2retro-hSyn-FLPo-T2A-GFP (a2). Neurons that are transduced by both AAV2retro-hSyn-FLPo-T2A-GFP and one of the two FLP-dependent AAV vectors would express mCherry (a3 and a4). b–e Body weight (b), fat mass and lean mass (c), body length (d), and food intake (e) of female Ntrk2lox/lox mice injected with AAV2retro-hSyn-FLPo-T2A-GFP into the LPBN and either AAV2-Ef1a-fDIO-mCherry or AAV2-Ef1a-fDIO-mCherry-P2A-Cre into the PVH. n = 10 mice in the control group and 6 mice in the Cre group. Body weight data were analyzed using two-way ANOVA with Bonferroni post hoc tests; F(1, 126) = 488.7, P < 0.0001 for virus. Other data were analyzed with two-tailed unpaired t test; ****P < 0.0001 for fat mass and lean mass, **P = 0.0014 for body length, and **P = 0.004 for food intake. f Retrogradely labeling of PVH neurons. CTB594 (250 nl) and CTB488 (200 nl) were injected into the VMH and the LPBN, respectively (f1-3). Some retrogradely labeled neurons are in the PVH (f4-7). Error bars indicate SEM. Scale bars represent 50 μm. Source data are provided as a Source Data file.

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