Fig. 4: Effects of mutations on cleavage rate at 10 mM GlcN6P. | Nature Communications

Fig. 4: Effects of mutations on cleavage rate at 10 mM GlcN6P.

From: Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

Fig. 4

a Heatmap of rates for all measured single and double mutants, with the first constituent point mutation indicated on the ordinate and the second on the abscissa. Sequence regions participating in duplex structures are labeled and color-coded as in Fig. 1. Heatmap of cleavage rates of all single mutants and basepaired-partner double mutants superimposed on the ribozyme tertiary (b) and secondary (c) structure, with secondary elements indicated in the same color scheme as a. The structure in b is based on PDB 2Z75. Source data are available in the Source Data file.

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