Fig. 5: Double-mutant rescue interactions (for 10 mM GlcN6P).
From: Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

a Heatmap of double-mutant rescue, as defined in the main text. Sequence regions participating in duplex structures are labeled and color-coded, as in Fig. 4. b–f Examples of rescue interactions, illustrating different candidate mechanisms: secondary-structure rearrangements (b, e), tertiary contacts seen in crystal structures (d, f), and novel tertiary interactions (c). The crystal structure in c, d, and f is PDB 2Z75. Source data are available in the Source Data file.