Fig. 4: The effectorness gradient is preserved after T cell activation. | Nature Communications

Fig. 4: The effectorness gradient is preserved after T cell activation.

From: Single-cell transcriptomics identifies an effectorness gradient shaping the response of CD4+ T cells to cytokines

Fig. 4

a Th0-stimulated TN and TM cells ordered in a branched pseudotime trajectory. Cells are colored by cell type (top panel) or effectorness (bottom panel). b Heatmap of the most variable genes along the Th0 pseudotime trajectory (Methods). The x axis represents cells ordered by effectorness (from left to right) and colors correspond to the scaled (Z-scored) expression of each gene in each cell. c Mapping of Th0-stimulated cells to their corresponding resting T cell populations. Integration of resting and Th0-stimulated cells using canonical correlation analysis (Methods) followed by UMAP embedding. Cells are colored by activation status (resting vs Th0, top panel) or cell type (bottom panel). d Density plots highlighting the location of cell clusters as defined in resting state. e UMAP embedding color-coded by the effectorness values of resting and stimulated cells. Source data are provided as a Source Data file.

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