Fig. 5: The effectorness gradient persists after cytokine-induced T cell polarization. | Nature Communications

Fig. 5: The effectorness gradient persists after cytokine-induced T cell polarization.

From: Single-cell transcriptomics identifies an effectorness gradient shaping the response of CD4+ T cells to cytokines

Fig. 5

a UMAP embedding of stimulated T cells into a two-dimensional space. Cells are colored by cell type (top panel) or effectorness value (bottom panel). b Density plots highlighting cells based on the cytokine stimulations. c Expression of selected cytokine-specific markers obtained from our bulk RNA-seq analysis. Cells are colored by their expression level of each marker gene. d UniFrac distances between TN and TM cells exposed to different cytokines summarized in a correlation plot. e Annotation of 17 cell clusters identified from unsupervised clustering using the top variable genes. Each cluster is annotated based on either the genes with highest expression or the effectorness and cytokine condition of the cells contained in it. b Heatmap of the top 10 markers of each cluster (Wilcoxon rank sum test). Colors encode the mean expression of each gene in each cluster. Labels were added to a number of example genes for each cluster. Source data are provided as a Source Data file.

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