Fig. 2: RAMS11 promotes an invasive phenotype.
From: Long non-coding RNA RAMS11 promotes metastatic colorectal cancer progression
Expression of RAMS11 in a LoVo CRISPR KO, SW620 silenced cells, and b HT29 overexpressing cells (Clone1 and Clone2) as measured by qPCR. c Images of DAPI-stained LoVo RAMS11 CRISPR KO cells and SW620 siRNA silenced cells show decreased invasion compared with controls. HT29 cell lines overexpressing RAMS11 show increased invasion. d Images of LoVo RAMS11 CRISPR KO cell lines and SW620 cells transfected with RAMS11 siRNAs show decreased migration. HT29 cell lines overexpressing RAMS11 show increased migration. e, f Quantification of invaded (n = 4) and migrated cells (n = 3) in LoVo and SW620 cells. g, h Quantification of invaded (n = 3) and migrated cells (n = 2) in HT29 cells. i RAMS11 CRISPR KO cells decreased growth on soft agar (n = 2) and RAMS11 overexpressing cells (n = 6) increased growth on soft agar. j, k Quantification of soft agar cells. All data are presented as mean values ± s.d, analyzed by two-tailed paired t-test, and repeated more than two times. Bar = 25 μM, *p < 0.05, **p < 0.005, #p < 0.0005. Source data are provided as a Source Data File.
