Fig. 2: Antioxidants reduce Helicobacter-induced 8-oxoG and colon tumorigenesis.
From: Limiting oxidative DNA damage reduces microbe-induced colitis-associated colorectal cancer
a Colon length measured in 12-week-old Helicobacter-infected IL10−/− mice untreated or treated with L-NIL, NAC, or VitC. IL10−/− I indicates that mice were infected with both H. mastomyrinus and H. typhlonius. N = 57 mice were examined. b Acute and chronic inflammation were assessed histopathologically in Helicobacter-infected or uninfected IL10−/− mice. N = 17 mice were examined. c Polyp number was analyzed in the colon of 12-week-old Helicobacter-infected IL10−/−mice untreated or treated with L-NIL, NAC, or VitC. N = 57 mice were examined. d Polyps counted in c were graded according their size. e Immunofluorescence for 8-oxoG and MitoTracker in colon from Helicobacter-infected IL10−/− mice administered with L-NIL or NAC. Representative images of four independent experiments involving at least one mouse per group are depicted. Magnification 100×. Scale bar = 10 µm. Right panel: one dot represents the median intensity of fluorescence of 40 nuclei per mouse. Data in a, b, c and e were analyzed using the two-sided non-parametric t-test Mann–Whitney; *p < 0.05, **p < 0.01; ns nonsignificant.
