Fig. 6: The MMR is the dominant repair pathway of the 8-oxoG lesion in mouse and human colon.
From: Limiting oxidative DNA damage reduces microbe-induced colitis-associated colorectal cancer
a Top panel: schematic outlining the experimental interventions before immunofluorescence against 8-oxoG. Antibiotic cocktail was administered in drinking water for 3 weeks. Butyrate or PBS were administered for three consecutive days. Left panel: immunofluorescence for DAPI, 8-oxoG, and MitoTracker in colon from mice of the indicated treatments and genotypes. Magnification 100×. Scale bar = 10 µm. Right panel: quantification of 8-oxoG immunofluorescence. One dot represents the median intensity of fluorescence of 40 nuclei per mouse as shown in Supplementary Fig. 7a. N = 24 samples were examined over four independent experiments. b Left panel: immunofluorescence for DAPI and 8-oxoG in colon from four Lynch syndrome patients. Magnification 63×. Scale bar = 10 µm. Right panel: quantification of 8-oxoG immunofluorescence are shown for the Lynch patients (b, left panel) and non-Lynch patients (Supplementary Fig. 8b). One dot represents the median intensity of fluorescence of 40 nuclei per patient as shown in Supplementary Fig. 8a. N = 12 biopsies (plus 12 matched normal tissue) were examined over two independent experiments. All data were analyzed using the two-sided non-parametric t-test Mann–Whitney; *p < 0.05.
