Fig. 4: Myc-directed transcriptional targets differ between tissues.

a The number of differentially expressed genes (DEGs) between organs. b Venn diagram depicting overlap of peaks within promoter regions identified by c-Myc ChIP sequencing from heart (red) or liver (green) isolated from R26^CMER/+ mice together with genes differentially expressed in response to Myc (UP DEGs—black, DOWN DEGs—grey). Areas are proportional to the numbers of promoters or DEGs within each cohort. c Venn diagram depicting numbers of genes showing increased expression in response to Myc (UP DEGs) in the kidney (yellow), liver (green), lung (blue) and heart (red); (FDR < 0.05 and abs(log2FC) > 0.5). d Six most significant GO Biological Process Gene sets that overlap with listed genes, showing a differential increase in expression (UP DEGs) in two or more tissues (kidney, liver, lung and heart). e Venn diagram depicting numbers of genes showing decreased expression in response to Myc (DOWN DEGs) in the kidney (yellow), liver (green), lung (blue) and heart (red). f Six most significant GO Biological Process Gene sets that overlap with listed genes that show a differential decrease in expression (DOWN DEGs) in two or more tissues (kidney, liver, lung and heart). g Three most significant Mouse Gene Atlas Gene sets that overlap with listed genes that show a differential decrease in expression (DOWN DEGs) only in the liver (liver specific). h Six most significant GO Biological Process Gene sets that overlap with listed genes that show a differential decrease in expression (DOWN DEGs) only in the liver (liver specific, absent in kidney, heart and lung). i Heatmap showing the union of DEGs called in each tissue; liver, lung, kidney and heart. Shown are mRNA expression fold changes (Log2) upon MycER^T2 activation relative to wild type. j Hearts isolated from R26^CMER/+ mice show enrichment of common Myc targets (exclusive to the liver, lung and kidney) in comparison to wild type (R26^+/+). All analysis determined by RNA sequencing performed in the heart (n = 5), kidney, lung and liver (n = 3) of R26^CMER/+ compared to wild-type (R26^+/+) mice at 4 h post administration of 4-OHT. Enrichr computed P-value (Fisher’s exact test), Z-score (modified Fisher’s exact) and combined score (C = ln(P) × Z) are shown.