Fig. 1: The generation of ancestor and evolved six-species hybrids.

Synthetic hybrid generation scheme using the iHyPr method. The example shown is the six-species hybrid yHRWh39. Chromosomes were colored according to their species designation, with height representing copy number, using the sppIDer pipeline48. For an extended explanation of iHyPr, including the other two crossing schemes, see Supplementary Figs. 1 and 2. Arrows mark hybridization steps. For additional intermediate and six-species hybrid nuclear and mitochondrial genomes with higher resolution, see Supplementary Figs. 3 and 4. Ancestor six-species hybrids underwent ALE for 80 generations.