Fig. 3: Characteristics of six-species hybrids.
a The number of species contributing genomes to synthetic hybrids is inversely correlated with the frequency of successful matings (n = 2 cross attempts). b Genome size is correlated with average cell area (average n = 63 counted cells). c Genome size (Supplementary Data 2) versus the average maximum growth rate (µ (n = 6 independent biological replicates), defined as (ln(OD2)−ln(OD1))/(T2–T1)) in rich medium at 20 °C (Supplementary Data 4). Dashed lines are the µ for the parent species indicated close to the line. For S. uvarum, the average of two strains with different HyPr plasmids is shown. d The maximum specific growth rate (µ, defined as (ln(OD2)−ln(OD1))/(T2−T1))) in rich medium at 20 °C is higher in interspecies hybrids inheriting S. cerevisiae mtDNA. Median values are represented by a horizontal line inside the box, and the upper and lower whiskers represent the highest and lowest values of the 1.5 × IQR (interquartile range), respectively. Colors correspond to the number of species contributing genomes to each strain. Synthetic hybrids generated from independent schemes are represented by different shapes in panels b–d. The Spearman rank-sum test R and p values are displayed. A linear regression and its 95% confidence interval are represented with a black dashed line and gray shadow, respectively. The mtDNA donor is underlined in the names in panel c. Species composition abbreviations are Scer, S. cerevisiae; Spar, S. paradoxus; Smik, S. mikatae; Sarb, S. arboricola; Skud, S. kudriavzevii; and Suva, S. uvarum. Source data are provided in the Source Data file and at http://bit.ly/2v1rq1T.
