Fig. 4: Genome reduction during hybrid construction and adaptive laboratory evolution.

a The genome contribution of each Saccharomyces species is stacked, and the percentage of retention is indicated inside the bar plot for each synthetic hybrid. Presence is reported, not copy number. For example, the red stacked bar for the hybrid yHRWh24 indicates that 46% of the total unique DNA for the S. cerevisiae parent (12.07 Mbp) was detected in the hybrid, which is ~5.55 Mbp of unique DNA. Synthetic hybrids are displayed in the order constructed (Supplementary Fig. 2). yHRWh4 is shown multiple times because of its use in two crossing schemes. We did not expect 100% genome contribution for each Saccharomyces species, even for recently created hybrids, because some genomic regions (e.g. repeats) are not unambiguously detectable with Illumina sequencing data. Genome size bars are colored according to each species’ contribution. The strain names are colored based on the mtDNA inheritance inferred from mitosppIDer (Supplementary Fig. 4), with two or more mtDNAs or regions shown as a gradient. b The nuclear compositions of the S. cerevisiae parent, synthetic hybrids, and evolved hybrids are plotted according to mtDNA inheritance (n = 1 sequenced strain). Hybrids with S. cerevisiae mtDNA or with other mtDNA are colored in red and light blue, respectively. Source data are in the Source Data file and at http://bit.ly/2v1rq1T.