Fig. 5: CHD3NEMO induces cell death in vFLIP-expressing PEL cell lines.
From: Modulation of virus-induced NF-κB signaling by NEMO coiled coil mimics
a CHD3NEMO but not CHD2NEMO or CHD4NEMO controls induces cell death in a panel of PEL cell lines. vFLIP (−) Namalwa cell line was used as a control. BC-3, BCBL-1, BC-1, and Namalwa cells were treated with increasing concentrations of CHD2NEMO, CHD3NEMO, CHD4NEMO, and cytotoxicity was assayed using the CellTiter-Glo assay at t = 24 h and t = 72 h, respectively. Data represents mean ± SEM of (n = 3) independent experiments performed in duplicates. The IC50s (µM) of the CHDs in the tested cell lines are displayed in the table below the graphs. b–c Flow cytometry analysis showing that CHD3NEMO induces apoptosis in vFLIP (+) BC-1 PEL cell line, but not vFLIP (−) Namalwa. CHD2NEMO and CHD4NEMO had no effect. BC-1 cells were treated with DMSO, 5 µM, 25 µM or 50 µM of CHD3NEMO for 48 h. After staining for DAPI and Annexin V, cells were examined using flow cytometry. Results were quantified into percentages of live (Annexin V−, DAPI−), early apoptotic cells (Annexin V+) or late apoptotic cells (Annexin V+/ DAPI + and DAPI+). Results shown are the mean ± SEM of (n = 3) independent experiments. Statistical analysis was performed using two-tailed unpaired t-test comparing treated samples to DMSO control. DMSO vs 25μM CHD3NEMO early apoptotic population p = 0.0124, DMSO versus 50 μM CHD3NEMO late apoptotic p = 0.0010 (*p ≤ 0.05, ***p ≤ 0.001 respectively). One-way ANOVA analysis was performed comparing 25 μM dose of each of CHD2NEMO, CHD3NEMO, CHD4NEMO peptides and was found to be significant with p = 0.0087, **p ≤ 0.01).
