Fig. 7: PBL13 and PIRE negatively regulate defense responses. | Nature Communications

Fig. 7: PBL13 and PIRE negatively regulate defense responses.

From: Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD

Fig. 7

a The expression of RBOHD in pbl13, pire, and pbl13::pire knockout lines. RBOHD expression was detected by anti-RBOHD immunoblotting. b Quantification of RBOHD abundance in different genetic backgrounds. Expression was normalized to Col-0. Statistical differences were determined by ANOVA with post-hoc Tukey HSD test, α = 0.05, n = 7 ± SD and letters indicate significant differences. c, d ROS burst c and bacterial titer d in Col-0, pbl13, pire, and pbl13::pire knockout lines. For the box-plot, whiskers indicate minimum and maximum values, line indicates the median, the box boundaries indicate the upper (25th percentile) and lower (75th percentile) quartiles, n > 23 for ROS burst and n = 18 for bacterial titers. Statistical differences were detected by ANOVA over all experiments with post-hoc Tukey HSD test, α = 0.01, and letters indicate significant differences. e Disease symptoms after Pto DC3000 infection on indicated genotypes 5 days post-inoculation (dpi).

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