Fig. 1: Landscape of chromatin organization in early SCNT embryo development.
From: Chromatin architecture reorganization in murine somatic cell nuclear transfer embryos
a Illustration of SCNT procedure and sample collections. According to the classical procedure of mouse cloning, donor nuclei from cumulus cells were injected into enucleated oocytes. Reconstructed embryos were chemically activated 1 h post-injection by culture in the Ca2+-free CZB medium including strontium chloride. b Hi-C produces a genome-wide contact matrix. The normalized Hi-C interaction frequencies (100-kb bin, chromosome 19) in each sample. Zoomed-in views (40-kb bin) are also shown. Each pixel represents all interactions between a 100-kb (40-kb) locus and another 100-kb (40-kb) locus; intensity corresponds to the ICE normalized value (0–10).
