Fig. 7: A schematic model showing the 3D chromatin structure dynamic during SCNT embryo development.
From: Chromatin architecture reorganization in murine somatic cell nuclear transfer embryos
a CCs exhibit interphase-state chromatin characterized by mature compartments and TADs, both of which are dissolved quickly after injection into enucleated oocytes. Consequently, 1-hpi and 1-hpa embryos exhibit a mitotic and secondary meiotic metaphase-like chromatin state lacking compartments and TADs, respectively. Compartments and TADs emerge in early-2-cell embryos, exist in later stages, and become mature in the ICM stage. TADs with enriched H3K9me3 signals are resistant to reprogramming. The differential reprogramming of chromatin architecture results in the loss of SE–P loops of regulating genes critical to development.
