Fig. 2: Tryptophan fluorescence quenching of TbGMPR W115R by purine nucleotides. | Nature Communications

Fig. 2: Tryptophan fluorescence quenching of TbGMPR W115R by purine nucleotides.

From: Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

Fig. 2

a–c (Upper panels) The fluorescence emission spectra of Trp120 in TbGMPR W115R. GTP (a), GMP (b), and ATP (c) were used as ligands. a–c (Lower panels) The peak value of each plot was re-plotted against the ratio of the ligand to protein concentration shown in logarithmic scale. Plots were fitted to the Hill equation. Data were obtained from three independent experiments (n = 3). Each data point represents a mean ± s.d. in error bars. Source data are provided as a Source Data file.

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