Fig. 5: SEC-SAXS analysis of TbGMPR in the presence of purine nucleotides. | Nature Communications

Fig. 5: SEC-SAXS analysis of TbGMPR in the presence of purine nucleotides.

From: Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

Fig. 5

a SAXS intensities for TbGMPR in the absence (black) or presence of GMP (green), GTP (blue), or ATP (red) were plotted against the scattering vector q defined as 4πsinθ/λ. The scattering intensity I(q) was represented in absolute intensity. The fitting curves were calculated by OLIGOMER. The inset shows the Guinier plots of each curve. b Dimensionless Kratky plots of TbGMPR in the presence of purine nucleotides. The plots were calculated for TbGMPR in the absence (gray) or presence of GMP (green), GTP (blue), or ATP (red). Cross-hair marks the Guinier–Kratky point (1.732, 1.1), the main peak position for globular particles. The inset shows the enlarged view of the peak position. Data were obtained from three independent experiments (n = 3). Source data are provided as a Source Data file.

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