Table 1 Kinetic data for TbGMPR wild-type (WT) and TbGMPR∆CBS.

From: Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

TbGMPR

Ligand

K0.5 (µM)

kcat (min−1)

kcat/K0.5 (105 M−1 min−1)

nHill

WT

None

184 ± 3

16.7 ± 0.15

0.913

3.04 ± 0.12

WT

GTP

49.4 ± 8.9

21.5 ± 1.68

4.36

1.00 ± 0.34

WT

ATP

1200 ± 12

13.4 ± 0.10

0.111

2.72 ± 0.07

∆CBS

None

162 ± 36

2.29 ± 0.20

0.141

1.00 ± 0.14

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