Fig. 4: Lifespan extension is mediated by loss of Per specifically in the intestine.
From: Circadian regulation of mitochondrial uncoupling and lifespan
Tissue-specific rescue of Per expression (dashed lines) in the per01 background (green) and controls (gray). a Ubiquitous rescue of Per during adulthood was sufficient to revert per01 lifespan to control levels. While neuronal overexpression of Per (b) during adulthood did not revert per01 lifespan, intestinal overexpression of Per (c, d) was sufficient to revert per01 lifespan to control levels. e Rescue of Per specifically in intestinal stem cells (ISCs) and enteroblasts (EBs) during development or adulthood reverted per01 lifespan to control levels. f Loss of period through ubiquitous CRISPR-mediated deletion during adulthood extended lifespan of otherwise wild-type flies, with no further lifespan extension in per01 nulls. CRISPR-mediated deletion of period in g the intestine or h IScs and EBs also extended lifespan. See Supplementary Table 1 for n and p values for lifespan experiments, particularly multicurve comparisons; p values were obtained by log-rank analysis.
