Fig. 5: Induction of ER stress and leptin deficiency promote autophagy genes. | Nature Communications

Fig. 5: Induction of ER stress and leptin deficiency promote autophagy genes.

From: The endoplasmic reticulum stress-autophagy pathway controls hypothalamic development and energy balance regulation in leptin-deficient neonates

Fig. 5

a Relative expression of autophagy-related protein (Atg) 5, Atg7, and Atg12 mRNA in mouse hypothalamic mHypoE-N43/5 treated with DMSO (control) or tunicamycin (0.1 µg/ml) for 5 h (n = 3–4 per group). b Representative images and quantification of microtubule-associated protein light chain 3 (LC3B)-immunoreactive puncta (red) in mouse hypothalamic mHypoE-N43/5 cells treated with DMSO (control) or tunicamycin (0.1 µg/ml) for 5 h (n = 4 per group). c Relative expression of Atg5, Atg7, and Atg12 mRNA in the arcuate nucleus (ARH) of postnatal day (P)10 wild-type (WT) mice and leptin-deficient (ob/ob) mice treated with either vehicle or tauroursodeoxycholic acid (TUDCA) neonatally (n = 4 per group). d, e Relative expression of Atg5, Atg7, and Atg12 mRNA in (d) sorted Pomc-GFP cells (n = 5 per group) and e the arcuate nucleus (ARH) of P0 WT (white bars) and ob/ob (gray bars) mice (n = 3–4 per group). f Representative images and quantification of LC3-GFP+ puncta (green) in the ARH of P10 WT (white bars) and ob/ob (gray bars) mice (n = 4 per group). Error bars represent the SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001 versus DMSO-treated cells (a, b); *P ≤ 0.05, ***P ≤ 0.001, and ****P ≤ 0.0001 versus vehicle-injected ob/ob mice (c); ****P ≤ 0.0001 versus Pomc-GFP mice (d); *P ≤ 0.05 versus LC3-GFP mice (f). Statistical significance between groups was determined using two-tailed Student’s t test (b, f) and two-way ANOVA (a, c, d, e) followed by Tukey’s multiple comparison test. ARH, arcuate nucleus of the hypothalamus. Scale bars, 100 µm (b), and 20 µm (f). Source data are provided as a Source Data file.

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