Fig. 6: Neonatal tauroursodeoxycholic acid treatment ameliorates metabolic defects in ob/ob; Pomc-Cre; Atg7loxP/loxP mice. | Nature Communications

Fig. 6: Neonatal tauroursodeoxycholic acid treatment ameliorates metabolic defects in ob/ob; Pomc-Cre; Atg7loxP/loxP mice.

From: The endoplasmic reticulum stress-autophagy pathway controls hypothalamic development and energy balance regulation in leptin-deficient neonates

Fig. 6

a Representative images and quantification of ubiquitin-binding protein (p62) immunoreactivity (red) in the arcuate nucleus (ARH) of 10-week-old wild-type (WT) mice, leptin-deficient (ob/ob) mice treated neonatally with vehicle or tauroursodeoxycholic acid (TUDCA), and ob/ob; Pomc-Cre; Atg7loxP/loxP mice treated neonatally with vehicle or TUDCA (n = 5–7 per group). b Pre- (ob/ob, ob/ob; Pomc-Cre; Atg7loxP/loxP + Vehicle: n = 9, ob/ob; Pomc-Cre; Atg7loxP/loxP + TUDCA: n = 8 per group) and (c) post-weaning growth curves of ob/ob mice and ob/ob; Pomc-Cre; Atg7loxP/loxP mice treated neonatally with vehicle or TUDCA (ob/ob, ob/ob; Pomc-Cre; Atg7loxP/loxP + Vehicle: n = 7, ob/ob; Pomc-Cre; Atg7loxP/loxP + TUDCA: n = 6 per group). d Blood glucose concentration after intraperitoneal injection of glucose and area under the glucose tolerance test (GTT) curve (AUC) (ob/ob, ob/ob; Pomc-Cre; Atg7loxP/loxP + TUDCA: n = 6, ob/ob; Pomc-Cre; Atg7loxP/loxP + Vehicle: n = 10 per group) and e serum insulin levels of 8-week-old fed ob/ob mice and ob/ob; Pomc-Cre; Atg7loxP/loxP mice treated neonatally with vehicle or TUDCA (n = 7 per group). f Food intake of 10-week-old ob/ob mice and ob/ob; Pomc-Cre; Atg7loxP/loxP mice treated neonatally with vehicle or TUDCA (n = 3–5 per group). g Body composition of 4- (n = 3 per group) and 10-week-old ob/ob mice and ob/ob; Pomc-Cre; Atg7loxP/loxP mice treated neonatally with vehicle or TUDCA (n = 4–5 per group). Error bars represent the SEM. *P ≤ 0.05, and **P < 0.01 versus all groups (a), *P ≤ 0.05, **P < 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001 versus vehicle-treated ob/ob; Pomc-Cre; Atg7loxP/loxP mice (b–g), #P < 0.05, ##P ≤ 0.01, ###P ≤ 0.001, and ####P ≤ 0.0001 versus WT mice (d). Statistical significance between groups was determined using one-way ANOVA (a, AUC in d, e–g) and two-way ANOVA (b–d) followed by Tukey’s multiple comparison test. ARH, arcuate nucleus of the hypothalamus; me, median eminence; V3, third ventricle. Scale bar, 50 µm (a). Source data are provided as a Source Data file.

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