Fig. 5: SOX9 plays a protective role during AKI.
To generate mice with renal tubule-specific Sox9 knockout, Ggt1-Cre mice were crossed with Sox9-floxed mice. a Representative western blots showing successful knockout in the renal tissues. Littermate control and Sox9 conditional knockout mice (indicated by Sox9PT−/−) were used to study the role of SOX9 in AKI. Bilateral renal ischemia was induced in wild-type and Sox9PT−/− mice for 30 min followed by examination of renal structure and function. b Blood urea nitrogen, c serum creatinine, d renal Kim1 mRNA expression, and e renal histological analysis (H&E) showed that tubular epithelial-specific Sox9 deficiency exacerbates ischemia-associated AKI. Data presented (b–e) are cumulative of three independent experiments (n = 6–7). Wild-type and Sox9PT−/− mice were treated with cisplatin (30 mg/kg) followed by examination of renal function. f Blood urea nitrogen, g serum creatinine, h renal Kim1 mRNA expression, i and renal histological analysis (H&E) showed that SOX9 regulates cisplatin-mediated AKI. Data presented (f–i) are cumulative of two out of four independent experiments (n = 8) that showed similar results. j Primary renal tubular cells were cultured from wild-type and Sox9-floxed mice. One week later, lentiviral transductions (Cre) were carried out to delete Sox9 gene. Western blot analysis confirmed SOX9 deletion. Blots are representative of two independent experiments. k Primary renal tubular cells with indicated genotype were treated with 50 µM cisplatin, followed by cell viability assessment using trypan blue staining. Data are presented as individual data points (n = 4 biologically independent samples), from one out of three independent experiments, all producing similar results. In all the bar graphs, experimental values are presented as mean ± s.e.m. The height of error bar = 1 s.e. and p < 0.05 was indicated as statistically significant. One-way ANOVA followed by Tukey’s multiple-comparison test was carried out, and statistical significance is indicated by *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
