Fig. 4: NK cells modulate microglia phenotype in hSOD1^G93A mice.

a Quantification of soma and scanning domain area, branch numbers and length of Iba1⁺ cells in slices obtained from the spinal cord of wt and hSOD1^G93A mice (13 weeks) treated with vehicle or Ab-NK1.1. Right: representative images (original magnification, ×600; Scale bar: 10 μm) of the maximal intensity projections of confocal z-stack images of Iba1⁺ cells in the different conditions, converted to binary images and then skeletonized. (n = 3 mice per treatment, 30 cells per mice in at least six slices. **P < 0.01 power 0.921 one-way ANOVA). Error bars show mean ± SEM. b Mean (±s.e.m.) area of Iba1⁺ cells (expressed as % of spinal cord area) in wt or hSOD1^G93A mice (13 weeks) treated with vehicle or Ab-NK1.1 (n = 4, **P < 0.01 power 1 one-way ANOVA). Right: representative immunofluorescence image of Iba1⁺ cells in spinal cord sections. Scale bar: 50 μm. c–g RT-PCR of il-6, il-1β, tnfα, nos2, nox2, p47phox, chil3, socs3, arg-1, tgfb, msod1, p2yr12, trem2, kcnn4, bdnf, il-15 gene expression in microglial cells isolated from the lumbar spinal cord of wt or hSOD1^G93A mice (13 weeks) treated with vehicle or Ab-NK1.1 (n = 5 mice. Data are the mean ± s.e.m. *P < 0.05 **P < 0.01 power >0.85 one-way ANOVA). Source data are provided as a Source Data file.