Fig. 4: The inhibition mechanism of MavC by Lpg2149. | Nature Communications

Fig. 4: The inhibition mechanism of MavC by Lpg2149.

From: Structural insights into the mechanism and inhibition of transglutaminase-induced ubiquitination by the Legionella effector MavC

Fig. 4

a Overall structure of MavC/Lpg2149. MavC is colored as in Fig. 1a and Lpg2149 is colored in blue. b, c Detailed interface of MavC–Lpg2149 interaction as marked in a, including A3 b and B3 c. d Isothermal titration calorimetry (ITC) assays to test binding of the Lpg2149 mutants to MavC. Representative binding curves and binding. affinities are shown. e Mutations that disrupt MavC–Lpg2149 interactions affect the inhibition capacity of Lpg2149. Lpg2149 and the mutants were incubated with MavC, UBE2N, and Ub for 2 h at 37 °C. Then the samples were treated as in Fig. 1i. Source data are provided as a Source Data file. The experiment was repeated independently three times with similar results.

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