Table 1 Performance summary of TES and tunable NOT gate.

Device	Design	Dynamic range (a.u.)		Fold change		Intersection		K range (RPU)
Device	Design	Low	High	Low	High	Low	High	K range (RPU)
TES	Original	333 ± 53	877 ± 695	14 ± 1.7	2.4 ± 1.2	0.78 ± 0.06	0.69 ± 0.16	–
	sRNA booster	538 ± 51	2064 ± 1070	227 ± 297	5.7 ± 1.8	0.46 ± 0.04	0.35 ± 0.15	–
	Noninsulated	882 ± 134	2149 ± 409	445 ± 412	31 ± 16	0.26 ± 0.07	0.27 ± 0.06	–
	Combined	1550 ± 209	1712 ± 584	1236 ± 613	66 ± 54	0.15 ± 0.04	0.22 ± 0.04	–
NOT gate	Original	17280 ± 1273	3512 ± 286	6.0 ± 0.1	1.5 ± 0.1	0.19 ± 0.04	0.84 ± 0.02	0.01–0.07
	sRNA booster	22040 ± 1601	2170 ± 654	5.8 ± 0.3	0.9 ± 0.3	0.13 ± 0.07	0.85 ± 0.02	0.01–0.06
	Noninsulated	17466 ± 1926	4061 ± 827	6.8 ± 0.3	2.6 ± 0.4	0.11 ± 0.03	0.56 ± 0.08	0.02–0.04
	Combined	27751 ± 3104	2383 ± 165	6.0 ± 0.6	0.9 ± 0.1	0.08 ± 0.05	0.90 ± 0.03	0.003–0.02

Average values are shown ±1 standard deviation calculated from flow cytometry data for three biological replicates. The low and high columns correspond to experiments when the tuner promoter activity is 0.002 RPU and 2.61 RPU, respectively. Dynamic range is calculated as the absolute difference in YFP fluorescence between on and off inputs states. The on and off input states correspond to input promoter activities of 6.6 RPU and 0.002 RPU for the TES, and 1.5 RPU and 0.002 RPU for the NOT gate, respectively. Fold change is calculated for YFP fluorescence between on and off input states. Intersection is calculated as the fractional overlap between distributions for on and off input states. The K range gives the span of K values from Hill functions fitted to experimental data. The designs are as follows: original designs are the initial constructs (Figs. 1a and 2a), sRNA booster designs include the additional sRNA booster plasmid (Fig. 3c), the noninsulated designs have the RiboJ element removed (Fig. 4), and the combined designs have both RiboJ removed and the sRNA booster plasmid present.

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