Fig. 1: CNS region-independent regulation of angiogenesis by oligodendroglial HIFα.
From: Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways
a Representative images of blood vessels labeled by the basement membrane marker Laminin in the spinal cord of Cnp-Cre:Hif1αfl/fl:Hif2αfl/fl and non-Cre littermate controls at postnatal day 14 (P14). Scale bars = 100 μm. b Percentage of Laminin+ area among total assessed area. Two-tailed Student’s t test with Welch’s correction, t(4.618) = 4.767, n = 5 each group. c RT-qPCR assay of Pecam1 mRNA (a.k.a. CD31), a marker of endothelial cells (ECs), in the P14 spinal cord. Two-tailed Student’s t test, t(8) = 2.526, n = 5 each group. d–f Representative images of immunohistochemical staining of ERG (a nuclear marker of ECs), isolectin B4 (IB4, labeling blood vessel basement membrane), and EdU (labeling actively dividing cells) and densities of marker positive cells (# per mm2) in Cnp-Cre, Hif1αfl/fl, Hif2αfl/fl and littermate control mice at P14. Two hours EdU pulse labeling. Two-tailed Student’s t test, ERG+, t(6) = 5.200 cortex, t(6) = 6.358 spinal cord; ERG+EdU+, t(6) = 4.496 cortex, t(6) = 6.365 spinal cord. n = 4 each group. g Percentage of ERG+ ECs that are EdU+. Two-tailed Student’s t test, t(6) = 4.089 cortex, t(6) = 3.181 spinal cord. n = 4 each group. h Representative images and quantification of Laminin in the spinal cord of P8 Cnp-Cre, Vhlfl/fl, and non-Cre control mice. Two-tailed Student’s t test, t(5) = 8.831. n = 4 Ctrl, 3 VHL cKO. i Densities (per mm2) of ERG+ ECs and ERG+EdU+ proliferating ECs in the P8 spinal cord. Two-tailed Student’s t test, t(5) = 11.10 ERG+, t(5) = 9.981 ERG+EdU+. n = 4 Ctrl, 3 VHL cKO. j RT-qPCR of Pecam1 mRNA in P14 spinal cord. Two-tailed Student’s t test with Welch’s correction, t(3.081) = 5.941. n = 6 Ctrl, 4 VHL cKO. k Representative images and quantification of Laminin in P5 Sox10-Cre, Vhlfl/fl and non-Cre controls. Two-tailed Student’s t test, t(6) = 3.625 spinal cord, t(6) = 3.428 cortex. n = 5 Ctrl, 3 VHL cKO. l RT-qPCR of Pecam1 mRNA in P5 spinal cord. Two-tailed Student’s t test with Welch’s correction, t(4.309) = 3.257. n = 8 Ctrl, 5 VHL cKO. m Densities (per mm2) of ERG+ ECs at P5. Two-tailed Student’s t test, t(6) = 6.964 cortex, t(6) = 5.324 spinal cord. n = 5 Ctrl, 3 VHL cKO. Scale bar: a, k 100 μm; d, h 50 μm. Data are shown as mean ± s.d. Source data of b, c, e, m are provided as a Source Data file.
