Fig. 2: Oligodendroglial HIFα does not activate Wnt/β-catenin signaling.
From: Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways
a RT-qPCR assay of mRNA levels of HIFα target gene Hk2 and Wnt/β-catenin target genes Axin2, Naked1, and Notum. Two-tailed Student’s t test, t(6) = 3.936 Hk2, t(6) = 1.275 Axin2 at P2; t(5) = 0.1307 Axin2 at P8; t(8) = 0.4906 Axin2, t(8) = 0.8386 Naked1, t(8) = 1.821 Notum at P14 spinal cord; t(8) = 1.736 Axin2, t(8) = 0.5617 Naked1 at P14 forebrain. n = 6 Ctrl, 4 VHL cKO. b Western blot assay of total β-catenin, active β-catenin, and Axin2. Two-tailed Student’s t test, t(4) = 1.247 β-catenin, t(4) = 0.5869 active β-catenin, t(4) = 0.614 Axin2. n = 3 each group. c RT-qPCR of Wnt7a mRNA. Two-tailed Student’s t test, t(6) = 0.960 P2 spinal cord, t(6) = 0.3724 P2 forebrain, t(8) = 1.736 P8 spinal cord. n = 4 each group at P2, n = 6 Ctrl, 4 VHL cKO at P8. d RT-qPCR of lacZ mRNA in Wnt/β-catenin reporter mice (BAT-lacZ) that had been crossed onto Cnp-Cre, Vhlfl/fl and non-Cre control backgrounds. Two-tailed Student’s t test, t(8) = 0.6072. n = 6 BAT-lacZ, 4 Cnp-Cre,Vhlfl/fl,BAT-lacZ. e, f RT-qPCR assay of mRNA level of Hk2, Axin2, Naked1, Wnt7a, and Wnt7b in the spinal cord of Sox10-Cre:Vhlfl/fl and non-Cre controls at P5. Two-tailed Student’s t test, t(11) = 7.011 Hk2, t(11) = 0.7422 Axin2, Welch’s corrected t(4.345) = 0.392 Naked1, t(11) = 0.3216 Wnt7a, Welch’s corrected t(4.709) = 0.4827 Wnt7b. n = 8 Ctrl, 5 VHL cKO. g RT-qPCR of HIFα target gene Hk2 and EC marker Pecam1 in P14 spinal cord. Two-tailed Student’s t test, t(4) = 4.158 Hk2, t(4) = 3.231 Pecam1. n = 3 each group. h Percent of laminin-occupying area among total area in the cerebral cortex at P14. Two-tailed Student’s t test, t(4) = 3.724. n = 3 each group. i Expression of Wnt/β-catenin target gene Axin2 and Sp5 in the spinal cord at P14 quantified by RT-qPCR. Two-tailed Student’s t test, t(4) = 0.5021 Axin2, t(4) = 2.006 Sp5. n = 3 each group. j, k RT-qPCR assay of mRNA levels of HIFα target genes and Wnt signaling genes in primary OPCs isolated from the neonatal brain of Sox10-Cre, Vhlfl/fl and non-Cre control mice. Two-tailed Student’s t test, Welch’s corrected t(6.282) = 6.065 Hk2, Welch’s corrected t(7.898) = 12.92 Ldha, Welch’s corrected t(6.199) = 4.009 Glut1, t(11) = 4.654 Pkm2, t(11) = 0.7772 Axin2, t(11) = 0.4936 Naked1, t(11) = 1.394 Wnt7a. n = 6 Ctrl, 7 VHL cKO. Data are shown as mean ± s.d. Source data of a–k are provided as a Source Data file.
