Fig. 5: Blocking Wnt secretion from oligodendrocyte progenitor cells (OPCs) does not affect HIFα-regulated CNS angiogenesis.
From: Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways
a, b Representative confocal images of Laminin-labeled blood vessels and ERG-labeled ECs in each group of mice. Scale bars = 50 µm. c, d Percent of Laminin-occupying area among total assessed area. Welch’s ANOVA followed by unpaired t test with Welch’s correction, *P < 0.05, ***P < 0.001, ns, not significant. W(2,5.256) = 30.38, P = 0.0013 for cortex. One-way ANOVA followed by Tukey’s multiple comparisons, **P < 0.01, ns, not significant. F(2,10) = 12.67, P = 0.0018 for spinal cord. n = 4 Ctrl, 5 VHL cKO, 4 VHL/WLS cKO. e, f Densities (#/mm2) of ERG+ ECs. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant. F(2,8) = 39.80, P < 0.0001 cortex, F(2,8) = 10.30, P = 0.0064 spinal cord. Mice from the above three groups were injected with tamoxifen at P1, P2, and P3 and sacrificed at P8. n = 4 Ctrl, 3 VHL cKO, 4 VHL/WLS cKO. Data are shown as mean ± s.d. Source data of c–f are provided as a Source Data file.
