Fig. 6: Oligodendroglial HIFα regulates VEGFA expression.
From: Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways
a RT-qPCR assay of Vegfa mRNA in the spinal cord of P14 mice. Two-tailed Student’s t test, t(6) = 2.590. n = 4 Ctrl, 4 HIFα cKO. b ELISA measurement of VEGFA concentration in the culture medium of primary OPCs isolated from neonatal forebrain of indicated genotypes. Two-tailed Student’s t test, t(8) = 4.208. n = 4 Ctrl, 6 HIFα cKO. c RT-qPCR assay of Vegfa mRNA in the spinal cord. Two-tailed Student’s t test, t(6) = 4.127 at P2; Welch’s correction t(3.139) = 4.299 at P14. n = 4 each group. d1–d4 Vegfa mRNA in situ hybridization in the spinal cord of P8 transgenic mice. Arrowheads point to Vegfa mRNA+ cells. Note that Vegfa mRNA signals were higher in the Cnp-Cre, Vhlfl/fl spinal cord (d2) than those in the non-Cre controls (d4). e1–e2 Vegfa and Plp dual fluorescent mRNA in situ hybridization in P8 spinal cord. Boxed areas were shown at higher magnification in single color channels. Note that Vegfa mRNA signals are higher in Plp+ oligodendrocytes in Cnp-Cre, Vhlfl/fl mutants than those in non-Cre controls. Scale bars: 10 µm. f RT-qPCR assay of Hk2 and Vegfa mRNA in the spinal cord of P14 Pdgfrα-CreERT2, Vhlfl/fl and non-Cre littermate controls that had been treated with tamoxifen (TM) at P6 and P7. Two-tailed Student’s t test, t(4) = 2.822 Hk2, t(4) = 4.770 Vegfa. n = 3 each group. g RT-qPCR assay of the mRNA levels of HIFα target genes Ldha, and Mct1 and Vegfa in the spinal cord of P14 Plp-CreERT2, Vhlfl/fl and non-Cre littermate controls that had been treated with tamoxifen at P6 and P7. Two-tailed Student’s t test, t(7) = 2.487 Mct1, t(7) = 7.194 Vegfa; Two-tailed Student’s t test with Welch’s correction, t(5.079) = 3.116 Ldha. h RT-qPCR assay of Vegfa mRNA in primary OPCs treated with HIFα stabilizer MOG and inhibitor Chetomin (cf Supplementary Fig. 9). Welch’s ANOVA followed by unpaired t test with Welch’s correction, **P < 0.01. W(2,5.270) = 79.93, P = 0.0012. n = 4 each group. i RT-qPCR assay of Vegfa mRNA in primary OPCs isolated from neonatal brains of Sox10-Cre, Vhlfl/fl mutants and littermate controls. Two-tailed Student’s t test, Welch’s-corrected t(7.008) = 12.38. n = 3 Ctrl, 7 VHL cKO. Data are shown as mean ± s.d. Source data of a–c, f–i are provided as a Source Data file.
