Fig. 7: Oligodendroglial HIFα regulates CNS angiogenesis through VEGFA-mediated signaling.
From: Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways
a–c RT-qPCR assay of the mRNA levels of Vegfa, Ldha, and Pecam1 in the spinal cord of each group of mice. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant. F(2,11) = 21.74, P = 0.0002 Vegfa; F(2,11) = 11.50, P = 0.0020 Ldha; F(2,11) = 20.31, P = 0.0002 Pecam1. n = 6 Ctrl, 4 VHL cKO, 4 VHL/VEGFA cKO. d, e Representative confocal images and quantification of Laminin-positive blood vessels. One-way ANOVA followed by Tukey’s multiple comparisons, **P < 0.01, ***P < 0.001, ns not significant. F(2,10) = 105.5, P < 0.0001 cortex, n = 5 Ctrl, 4 VHL cKO, 4 VHL/VEGFA cKO.; F(2,13) = 133.2, P < 0.0001 spinal cord, n = 5 Ctrl, 7 VHL cKO, 4 VHL/VEGFA cKO. Scale bars = 10 μm. f, g densities (#/mm2) of ERG+ ECs and ERG+BrdU+ proliferating ECs. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, **P < 0.01, ***P < 0.001. ERG+ ECs, F(2,10) = 113.1, P < 0.0001 spinal cord, n = 6 Ctrl, 4 VHL cKO, 3 VHL/VEGFA cKO; F(2,12) = 169.0, P < 0.0001 cortex, n = 7 Ctrl, 5 VHL cKO, 3 VHL/VEGFA cKO; ERG+BrdU+ proliferating ECs, F(2,10) = 24.09, P = 0.0001 spinal cord, n = 5 Ctrl, 4 VHL cKO, 4 VHL/VEGFA cKO; F(2,12) = 86.14, P < 0.0001 cortex, n = 7 Ctrl, 5 VHL cKO, 4 VHL/VEGFA cKO. Data are shown as mean ± s.d. Source data of a–g are provided as a Source Data file.
