Fig. 9: Constitutively blocking Wnt secretion from astrocytes reduces HIFα-regulated angiogenesis in the early adult CNS.
From: Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways
a, b Western blot (a) and quantification (b) of the active form of β-catenin, Wnt/β-catenin target gene Axin2, EC-specific nuclear protein ERG in the spinal cord at P30. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, **P < 0.01, ***P < 0.001. F(2,6) = 13.38, P = 0.0061 active β-catenin, F(2,6) = 41.26, P = 0.0003 Axin2, F(2,6) = 65.59, P < 0.0001 ERG. n = 3 each group. c Representative confocal images of Laminin and IB4 in the spinal cord at P30, scale bars = 20 μm. d, e percentage of Laminin-occupying area among total area at P30. One-way ANOVA followed by Tukey’s multiple comparisons, *P < 0.05, ***P < 0.001. F(2,9) = 332.0, P < 0.0001 spinal cord, F(2,9) = 59.19, P < 0.0001 cerebral cortex. n = 5 Ctrl, 4 VHL cKO, 3 VHL/WLS cKO. f Representative confocal images of ERG and IB4 in the spinal cord at P30, scale bars = 20 μm. g, h Densities (per mm2) of ERG+ ECs at P30. One-way ANOVA followed by Tukey’s multiple comparisons, **P < 0.01, ***P < 0.001. F(2,10) = 124.1, P < 0.0001 spinal cord, F(2,9) = 219.2, P < 0.0001 cerebral cortex. n = 5 Ctrl, 5 VHL cKO, 3 VHL/WLS cKO. Data are shown as mean ± s.d. Source data of a, b, d, e, g, h are provided as a Source Data file.
