Fig. 1: Strategy for expanding the chemical space of a target bioactive natural product via a synthetic biology approach. | Nature Communications

Fig. 1: Strategy for expanding the chemical space of a target bioactive natural product via a synthetic biology approach.

From: Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones

Fig. 1

a Genome mining and reconstruction of the target natural product biosynthetic pathway. b Genome mining and reconstruction of the related pathways to produce natural analogues. c Pathway extension for combinatorial biosynthesis by adding additional enzymes to the native pathways to produce non-natural analogues (additionally modified analogues), Grey circles, blue circles and green diamonds show intermediates, natural analogues and non-natural analogues, respectively. Black, blue and green arrows show biosynthetic pathway for target natural product, its related pathways and extended pathways, respectively.

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