Fig. 5: DDPs show anti-CSC activity in breast cancer cell line MCF-7. | Nature Communications

Fig. 5: DDPs show anti-CSC activity in breast cancer cell line MCF-7.

From: Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones

Fig. 5

a Effect of DDPs on mammosphere formation. After the cells were cultured in the presence of 5 μM DDPs for 7 days, the number of mammospheres was microscopically counted and the percentage of mammosphere-forming cells was determined as mammosphere-forming efficiency (MFE; %). Data represent mean ± s.d. from three independent replicate experiments. P-values were calculated by a two-sided unpaired Student’s t-test, compared with control. b Micrographs show representative images of mammosphere formation from three independent replicate experiments using compound 11, 12, 16, 22. The scale bar, 50 mm. c Effect of DDPs (1 μM, 3 days) on ALDH-positive cells in MCF-7 cells. Data represent mean ± s.d. from three independent replicate experiments. P-values were calculated by a two-sided unpaired Student’s t-test, compared with control. d Dose-dependent effect of compound 12 on ALDH-positive cells in MCF-7 cells. Data represent mean ± s.d. from three independent replicate experiments. P-values were calculated by a two-sided unpaired Student’s t-test. Source data underlying Fig. 5a, c, d are provided in a Source Data file.

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