Fig. 1: Kinesin-1 regulates cross-presentation.

a Relative quantification of Kif5a, Kif5b and Kif5c transcripts by real-time PCR in CD8α⁺ and CD11b⁺ DCs and BMDCs from WT or cKO^Kif5b mice. Transcript levels for each sample are expressed relative to Kif5b. Graph shows mean ± S.E.M. (n = 3). b Contour plots of DCs from the spleen of either WT or cKO^Kif5b mice pregated on CD19⁻, Gr⁻, F4/80⁻ CD11c⁺, and IA/IE⁺. The data are representative of three independent experiments. c Absolute numbers of CD11b⁺ DCs and CD8α⁺ DCs in the spleen of WT (blue circles) and cKO^Kif5b (red squares) mice. The data are representative of five independent experiments. d–g The efficiency of cross-presentation of different concentration of sOVA and the presentation of OVA peptide SIINFEKL in vitro by CD8α⁺ DCs (d, g), CD11b⁺ DCs (e, g) and BMDCs (f, g) from WT (blue histogram or line) or cKO^Kif5b (red histogram or line) mice was measured as IL-2 secretion by OT-I T cells after 16 h of co-culture. Graph shows mean ± S.E.M. (n = 4). Statistical significance was determined by the two-way ANOVA and Sidak test’s correction for multiple comparison. *P < 0.05; **P < 0.005; ***P < 0.0001.