Fig. 4: Kinesin-1 regulates the intracellular trafficking of sOVA. | Nature Communications

Fig. 4: Kinesin-1 regulates the intracellular trafficking of sOVA.

From: Kinesin-1 regulates antigen cross-presentation through the scission of tubulations from early endosomes in dendritic cells

Fig. 4

BMDCs from WT or cKOKif5b mice were incubated for 15, 30 or 60 min with Alexa-Flour-647-OVA and then plated on glass coverslips. The cells were fixed, permeabilized and stained with anti-EEA1, anti-Lamp1 or anti-Rab11 antibodies. The left panel shown representative images for the 15 min time point observed in three independent experiments. Bars: 5 μm. n = 32 cells per condition. Statistical significance (shown in the right panel; WT blue circle, cKOKif5b red square) was determined by the two-way ANOVA and Sidak test’s correction for multiple comparison. **P < 0.005; ***P < 0.0001. Graphs show mean ± S.E.M.

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