Fig. 5: Kinesin-1 regulates MHC-I recycling.

a CD8α⁺, CD11b⁺ DCs and BMDCs from WT (blue line) or cKO^Kif5b (red line) mice were labelled for H-2K^b and analysed using FACS. The figure shows a FACS histogram that was representative of three independent experiments. b MHC-I recycling ability in CD8α⁺, CD11b⁺ DCs and BMDCs from WT (blue line) or cKO^Kif5b (red line) mice was measured using FACS at the indicated time points. Statistical significance was determined by the two-way ANOVA and Sidak test’s correction for multiple comparison. **P < 0.005; ***P < 0.0001. c TrfR (CD71) recycling ability was measured using FACS at the indicated time periods in CD8α⁺, CD11b⁺ DCs and BMDCs from WT or cKO^kif5b mice. Statistical analysis: *P < 0.05; **P < 0.005; ***P < 0.0001 in the two-way ANOVA and Sidak test’s correction for multiple comparison. d MHC-I recycling in the presence of primaquine in BMDCs from WT or cKO^Kif5b mice was measured using FACS at the indicated time points. Statistical analysis: **P < 0.005; in the two-way ANOVA and Sidak test’s correction for multiple comparison. b–d Graphs are representative of four independent experiments. e BMDCs from WT or cKO^kif5b mice were plated on glass coverslips and were fixed, permeabilized and stained with anti-EEA1, anti-Rab11 or anti-MHC-I antibodies. Representative images are shown in the left panel observed in three independent experiments. (n = 33 cells per condition upper panel, n = 29 cells per condition lower panel) Bars: 5 μm. Statistical analysis (shown in the right panel; WT blue circle, cKO^Kif5b red square): **P < 0.005; ***P < 0.0001 in an two-tailed unpaired Student’s t test. b–e Graphs show mean ± S.E.M.