Fig. 8: RPTEC-SNCA deletion aggravates adenine-induced renal fibrosis.

a–f, k Total mRNA was extracted from kidneys of PEPCK^Cre+ SNCA^wt/wt and PEPCK^Cre+ SNCA^fl/fl mice fed a regular or adenine rich diet for 4 weeks. mRNA levels of collagen I, fibronectin, α-SMA, vimentin, TGF-β1, cadherin-16 and SNCA were determined by quantitative real-time PCR and normalized to TBP. m Whole kidney lysates were processed for protein analysis and were immunoblotted with antibodies against α-SMA, vimentin, collagen I and GAPDH. m Representative Western blot analysis and g–i quantitative analysis by densitometry. j, l Quantification of collagen deposition in kidneys of PEPCK^Cre+ SNCA^wt/wt and PEPCK^Cre+ SNCA^fl/fl mice after Sirius red staining (j) and immunohistochemistry for collagen I (l) was expressed as a positive stained area vs. total analyzed area. Data are presented as mean ± SEM (n = 5–7 mice/group). *p < 0.05, **p < 0.01, ***p < 0.001. The p-value by two-way ANOVA. n Representative images of Sirius red staining and immunohistochemistry for α-SMA, FSP1 and collagen I in mouse kidneys. Scale bar represents 50 µm. Source data are provided as a Source Data file.