Fig. 3: pHluo_M153R-CD63-positive trails mark secreted exosomes.
From: A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
a Representative images of immunofluorescent staining of fixed cells for the exosomal markers TSG101 or Alix (magenta) and pHluorin (green). Arrows indicate colocalization of TSG101 (magenta) with pHluo_M153R-CD63-positive puncta (green) to form white. Note many white puncta in the CD63 + Alix merged images. pHluo_M-CD63, pHluorin_M153R-CD63. n = 29 and 39 images for TSG101 and Alix, respectively, from four independent experiments. Scale bar, 30 µm. b Colocalization analysis from (a) shown as a scatter-plot (median and quartile range). ***P < 0.001 by two-sided unpaired Mann−Whitney U test. c Correlative light-electron microscopy of pHluo_M153R-CD63-stably expressing HT1080 (green). Increasing magnification of images is shown to the right and indicated with i or ii. Representative of 22 cells from four experiments. SEM scanning electron microscopy. Source data are provided as a Source Data file.
