Fig. 5: Exosomes are deposited at the front of migrating cells on a nanopatterned dish.

a Time series from Supplementary Movie 5, showing trajectories of tracked exosome deposits under the cell on a nanopatterned dish. Colors on heat map represent the time elapsed since deposition occurred. Note that cyan color shows newly deposited exosomes. Note the image at the right bottom corner (Full Track) shows the overlap of all of the tracks from the movie. n = 23 cells from four independent experiments. Scale bar, 50 µm. b, c Analysis of mobility of exosome deposits from movies as in (a) with scatter dot plots in (b) (mean ± standard error) showing the percent of exosome deposits/cell with displacement length > or <2 µm, where each dot represents the median displacement length of exosome deposits in that category from each single cell. ***P < 0.001 by two-sided paired Student’s t test. c Total events (n = 34,052 from 23 cells) of displacement length are shown in a violin plot (median with interquartile range) from (a). Source data are provided as a Source Data file.