Fig. 3: Heat induces rearrangement of chromatin organization.
a Genome-wide chromatin organization revealed by a Hi-C interaction frequency heatmap at 100 kb resolution for all Arabidopsis chromosomes in Control and Heat. Each pixel denotes all interactions between any two 100 kb genomic loci from the linear genome. Intensity represents log2 normalized contact frequencies. The top track shows the positions of centromeres (black) and pericentromeres (gray). b Genome-wide heatmap of relative interaction differences between Heat and Control. The relative difference is calculated as the difference of two interaction frequencies divided by the mean of two interaction frequencies. In the plot, Hi-C interactions that become stronger (chromosome condensation) in Heat compared with Control are in red to orange, while Hi-C interactions that become weaker (chromosome loosening) in Heat compared with Control are in blue to green. Hi-C interactions that do not change in Heat compared with Control are in white. The genomic bin size is 100 kb and the upper- and lower-triangular matrices are symmetric. The top track shows the positions of centromeres (black) and pericentromeres (gray). c Averaged scaling plot of interaction frequencies against increasing genomic distance for all Arabidopsis chromosomes. The genomic bin size is 100 kb. d Averaged scaling plot of interaction frequencies against increasing genomic distance for all Arabidopsis chromosome arms. The genomic bin size is 100 kb. e Averaged scaling plot of interaction frequencies against increasing genomic distance for all Arabidopsis pericentromeric regions. The genomic bin size is 100 kb.
