Fig. 5: Reduced interactions among KEEs under heat stress.

a Pairwise interaction scores of all ten KEEs in Control and Heat, defined as the interactions per hundred square kilobases per billion mapped reads (ihskb). KEE3/KEE4 and KEE7/KEE8 are highlighted. For each box plot, center lines indicate the medians; boxes show the 25th and 75th percentiles; whiskers extend to the minimum and maximum. b Perspective plots of the interactions between KEE7 and KEE8 in Control and Heat. The X-axis and Y-axis show the genomic positions of KEE7 (Chr4: 11,050,000–11,206,537) and KEE8 (Chr4: 15,441,465–15,537,500). The Z-axis shows the interaction scores. c Triangle heatmaps of the relative differences between Heat and Control in chromosomes 3 and 4. Hi-C interactions that become stronger (chromosome condensation) in Heat than those in Control are in red to orange, while Hi-C interactions that become weaker (chromosome loosening) in Heat than those in Control are in blue to green. Hi-C interactions that do not change in Heat compared with Control are in white. The genomic bin size is 100 kb. The interactions of KEE3/KEE4 and KEE7/KEE8 are highlighted with circles. The bottom track shows the positions of centromeres (black), pericentromeres (gray), the knob hk4s (blue), and KEEs (red). d 3C results showing the significantly decreased interactions between KEE3 and KEE4 in Heat compared with that in Control, and the increases in Recovery compared with Heat. Circles denote relative interaction frequency. Bars indicate mean values of three technical replicates. e FISH results showing the association rates of KEE3/KEE4 and of KEE7/KEE8. Bars indicate the association rates (“Methods”). Significant differences between two groups at a time were determined by the two-sided Fisher’s exact test, with p values adjusted by the false discovery rate method for multiple comparisons. Exact p values are shown above the bars. Source data of d and e are provided as a Source Data file.